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Updated: Jan 29, 2026

Studying Protein Import into Chloroplasts Using Protoplasts
Published on: December 10, 2018
Bean (Phaseolus vulgaris L.) protoplasts as a model system to study the expression and stability of recombinant seed
G Giovinazzo1, V Greco1, A Vitale1
1Istituto Biosintesi Vegetali CNR, via Bassini 15, I-20133 Milano, Italy, , , , , , IT.
Abstract:
A suitable protocol for the transient expression of seed protein genes in protoplasts derived from cell suspension cultures of common bean has been established. Preliminary analyses of cultures to verify the synthesis of phaseolin - actively accumulated by the starting tissue, the developing cotyledon - showed that the protein was no longer synthesised after 5 days of culture. Transient expression of a phaseolin sequence, driven by a constitutive promoter, resulted in the accumulation of the correctly glycosylated and assembled protein. This system, when compared to tobacco protoplasts, largely avoids phaseolin fragmentation and the presence of contaminant polypeptides in the immunoprecipitates. Therefore, bean protoplasts are a good system to study the expression of wild-type as well as in-vitro-modified bean seed proteins.
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