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Updated: Jan 29, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Enhanced production of scopolin bySolanum aviculare cells immobilised within Ca-alginate gel beads
C Roisin1, F Gillet-Manceau2, J E Nava Saucedo1
1Laboratoire de Génie Cellulaire (EA 906), Université de Picardie Jules Verne, Faculté des Sciences, 33, rue St.-Leu, F-80039, Amiens Cedex, France.
Abstract:
Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour ofS. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics ofSolanum aviculare cells immobilised within Ca-alginate beads were notably different from those of suspended cells. ImmobilisedS. aviculare have accumulated scopolin (up to 120 μg·g-1 FWB) within beads and excreted it into the culture medium (up to 8 μg·g-1 FWB). Contrary to suspended cells which have accumulated only traces of this metabolite within intracellular compartments (1 μg·g-1 FWB), no scopolin has been found into the culture medium.
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