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Plasmid-Mediated Quinolone Resistance in Pseudomonas aeruginosa Isolated from Burn Patients in Tehran, Iran

Azam Molapour1, Amir Peymani2, Parvaneh Saffarain1

  • 1Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Infectious Disorders Drug Targets
|February 8, 2019
PubMed
Abstract

Insights

Pseudomonas aeruginosa from burn infections showed complete quinolone resistance due to the aac(6')-Ib-cr gene. This highlights the need to prevent the spread of these resistant bacterial clones.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Plasmid-mediated quinolone resistance is a growing global health concern.
  • Pseudomonas aeruginosa is a significant pathogen, particularly in burn wound infections.
  • Understanding resistance mechanisms is crucial for effective treatment.

Purpose of the Study:

  • To determine antibiotic susceptibility of P. aeruginosa from burn infections.
  • To identify the frequency of specific resistance genes (qepA, aac(6 times')-Ib, qnr).
  • To analyze the clonal types of resistant P. aeruginosa isolates.

Main Methods:

  • Isolation and identification of P. aeruginosa from burn wound samples.
  • Antibiotic susceptibility testing using Kirby-Bauer method.
  • Detection of resistance genes via PCR and sequencing.
  • Clonal typing using repetitive extragenic palindromic sequence-based PCR (REP-PCR).

Main Results:

  • All P. aeruginosa isolates (100%) exhibited complete resistance to quinolones.
  • The qnr and qepA genes were not detected.
  • The aac(6 times')-Ib gene was present in all isolates, identified as the aac(6 times')-Ib-cr variant.
  • REP-PCR identified three distinct clones (A, B, and C) among the resistant isolates.

Conclusions:

  • The aac(6 times')-Ib-cr variant is the primary mechanism of quinolone resistance in these P. aeruginosa isolates.
  • The qnr and qepA genes do not contribute to quinolone resistance in this context.
  • Clonal spread of resistant P. aeruginosa is significant in burn infections, necessitating infection control measures.
Keywords:
PCRPseudomonas aeruginosaaac(6`)-Ibgram-negative pathogensplasmid-mediated quinolone resistanceqepAqnr.

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