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Updated: Jan 29, 2026
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Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Published on: May 17, 2024
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Repeated human deciduous tooth-derived dental pulp cell reprogramming factor transfection yields multipotent
Miki Soda1, Issei Saitoh2, Tomoya Murakami1
1Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
Scientific Reports
|February 8, 2019
Summary
Researchers isolated human tissue-specific stem cells (hTSCs) from differentiated cells using reprogramming factors. Repeated transfections enhanced stem cell generation and multipotency, suggesting a novel method for stem cell isolation.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Cellular reprogramming
Background:
- Human tissue-specific stem cells (hTSCs) differentiate into multiple lineages.
- Induced TSCs from pancreas and hepatocytes show reduced tumor formation compared to iPSCs.
- hTSCs are hypothesized to exist in a partially reprogrammed state before iPSC transition.
Purpose of the Study:
- To investigate the isolation of hTSCs from terminally differentiated cells via transient reprogramming factor overexpression.
- To determine if hTSCs can be generated from human deciduous tooth-derived dental pulp cells (HDDPCs) and fibroblasts.
- To evaluate the multipotency and characteristics of HDDPCs after reprogramming.
Main Methods:
- Transfection of differentiated cells (HDDPCs and fibroblasts) with Yamanaka's reprogramming factors.
- Cytochemical staining for alkaline phosphatase (ALP) activity as a stem cell marker.
- Repeated transfections (up to 3 times) to enhance induced pluripotent stem cell (iPSC) generation.
Main Results:
- Increased ALP activity observed in a small percentage of HDDPCs and fibroblasts post-transfection.
- Repeated transfections improved iPSC generation efficiency.
- HDDPCs exhibited enhanced multipotency after two weeks compared to parental cells.
Conclusions:
- iPSC technology can be utilized to isolate TSCs from HDDPCs and fibroblasts.
- Transient reprogramming factor overexpression can isolate hTSCs from differentiated cells.
- Repeated reprogramming factor introduction can reverse differentiation and enhance stem cell properties.
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