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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Function of complement factor H and imaging of small molecules by MALDI-MSI in a methamphetamine behavioral
Jiamin Xu1, Zhilin Zhang1, Runzhe Liu1
1State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Behavioural Brain Research
|February 8, 2019
Summary
Methamphetamine (METH) addiction involves changes in the complement system, specifically increased complement factor H (CFH) and decreased C1q in the brain. This study reveals oxidative stress and altered energy metabolism during METH addiction.
Area of Science:
- Neuroscience
- Immunology
- Pharmacology
Background:
- Methamphetamine (METH) abuse is a significant public health issue, with its harms potentially exceeding those of traditional opioids.
- Previous research indicated elevated complement factor H (CFH) in METH-addicted individuals and animal models.
Purpose of the Study:
- To investigate the role of CFH and C1q in the neurobiological mechanisms underlying METH addiction.
- To analyze the distribution of small molecules in key brain regions affected by METH addiction.
Main Methods:
- Utilized ELISA and immunofluorescence to assess CFH and C1q levels in a METH behavioral sensitization mouse model.
- Employed matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) to map small molecules in the nucleus accumbens, hippocampus, and ventral tegmental area.
Main Results:
- Confirmed upregulation of CFH in serum and hippocampus of METH-treated mice.
- Observed a significant decrease in C1q within the hippocampus.
- MALDI-MSI revealed alterations in potassium ions, antioxidants, neurotransmitters, and ATP metabolism-related molecules.
Conclusions:
- The complement system plays a role in METH addiction.
- Evidence of oxidative stress and altered energy metabolism supports the involvement of these pathways in METH addiction.
- Further research into these mechanisms is warranted.
Keywords:
Behavioral sensitization modelC1qComplement factor HMatrix-assisted laser desorption time of flight ionization mass spectrometry imaging (MALDI–TOF–MSI)MetabolitesMethamphetamineMore Related Videos
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