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Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses
Published on: March 29, 2017
Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in
Zhenglu Xie1, Yang Wang1, Juqing Huang2
1Jinshan College of Fujian Agriculture and Forestry University, No. 15 Shangxiadian Road, Cangshan District, Fuzhou City, Fujian Province 350002, PR of China.
Abstract:
Inflammatory response remains one of the most common and serious complications of disease. In order to profound understanding relationship between Phellinus linteus polysaccharides (TCM) and inflammation, inflammatory cell model was constructed by LPS acting RAW264.7 cell line. The results showed that TCM could decrease the pro-inflammatory cytokines (TNF-α, IL-1β, IL-2, IL-6 and IL-12) contents and the mRNA expression levels and increase the anti-inflammatory cytokines (IL-4 and IL-10) contents and the mRNA expression levels. Additionally, the levels of NF-κB translocation was significantly decreased, which associated with the IκBα phosphorylation level decreased and the AMPKα phosphorylation level increased. These results indicated that TCM could reduce the inflammatory responses in the LPS induced inflammatory cell model might be related to inhibit NF-κB translocation and regulate the balance of anti-inflammatory and pro-inflammatory factors.
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