Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Characteristics of Practical Op Amps01:16

Characteristics of Practical Op Amps

860
A difference amplifier, a crucial component in numerous electronic devices, ideally amplifies only the difference-mode signal, which is the difference between two input signals. However, in practical circuits, the output voltage depends on both the differential gain and the common-mode gain.
The ratio of differential gain to the common-mode gain is defined as the common-mode rejection ratio (CMRR). This ratio quantifies the ability of operational amplifiers (op-amps) to reject common-mode...
860
Theoretical Foundations of Nursing Practice01:30

Theoretical Foundations of Nursing Practice

17.4K
Theories play an essential role in organizing patient care. Theories refer to a proposed or followed belief, policy, or procedure that is the basis for action. Nursing theories are knowledge-based concepts that guide nurses' actions, influence nursing education and practice, and allow nurses to care for their patients.
Theories provide a perspective to assess patients' conditions and organize data and methods. They also assist in analyzing and interpreting information. They represent a...
17.4K
Equivalent Circuits for Practical Transformers01:28

Equivalent Circuits for Practical Transformers

1.4K
The practical equivalent circuits of single-phase two-winding transformers exhibit significant deviations from their idealized versions due to the inherent properties of winding resistance and finite core permeability. These properties result in real and reactive power losses, affecting the transformer's performance. Understanding these deviations is crucial for designing more efficient transformers.
In a practical transformer, each winding exhibits resistance and leakage reactance. The...
1.4K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.3K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.3K
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

1.2K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
1.2K
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

1.1K
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimentally induced immunity in gulls to type E botulism.

American journal of veterinary research·1967
Same author

FACTORS AFFECTING THE DEVELOPMENT OF CLOSTRIDIUM BOTULINUM IN WHOLE MILK.

Applied microbiology·1965
Same author

DEVELOPMENT OF CLOSTRIDIUM BOTULINUM TYPE 62A IN A TRYPTICASE MEDIUM AUTOCLAVED IN THE PRESENCE OF LACTOSE.

Journal of dairy science·1965
Same author

DEVELOPMENT OF CLOSTRIDIUM BOTULINUM IN EXPERIMENTALLY INOCULATED STERILE SKIM MILK HELD AT LOW TEMPERATURES.

American journal of public health and the nation's health·1964
Same author

Effect of heat treatment on the growth of surviving cells.

Journal of bacteriology·1959
Same author

CIRCULIN AN ANTIBIOTIC FROM AN ORGANISM RESEMBLING BACILLUS CIRCULANS.

Journal of bacteriology·1949

Related Experiment Video

Updated: Jan 29, 2026

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.1K

A Practical Sensitive Test to Detect Penicillin in Milk.

O W Kaufmann1

  • 1Cincinnati Training Facility, Division of Federal-State Relations EDRO, Food and Drug Administration, Room 8002 Federal Building 550 Main Street, Cincinnati, Ohio 45202.

Journal of Food Protection
|February 9, 2019
PubMed
Summary
This summary is machine-generated.

A modified International Dairy Federation disc assay offers a sensitive and rapid method for detecting penicillin in milk. This enhanced method can detect as little as 0.002 units of penicillin per milliliter, ensuring milk safety.

More Related Videos

Milk Collection Methods for Mice and Reeves' Muntjac Deer
07:34

Milk Collection Methods for Mice and Reeves' Muntjac Deer

Published on: July 19, 2014

20.6K
Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
04:35

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles

Published on: November 14, 2025

371

Related Experiment Videos

Last Updated: Jan 29, 2026

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.1K
Milk Collection Methods for Mice and Reeves' Muntjac Deer
07:34

Milk Collection Methods for Mice and Reeves' Muntjac Deer

Published on: July 19, 2014

20.6K
Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
04:35

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles

Published on: November 14, 2025

371

Area of Science:

  • Food Science
  • Microbiology
  • Analytical Chemistry

Background:

  • Penicillin contamination in milk poses a public health risk.
  • Accurate and rapid detection methods are crucial for milk quality control.
  • Existing methods may lack sensitivity or speed.

Purpose of the Study:

  • To modify and optimize the International Dairy Federation (IDF) disc assay for penicillin determination in milk.
  • To enhance the sensitivity and reduce the assay time for penicillin detection.
  • To provide a reliable method for ensuring milk safety and quality.

Main Methods:

  • Modification of the standard IDF disc assay protocol.
  • Optimization of seed broth preparation, seeded plate creation, and standard solution formulation.
  • Incubation of plates at 55°C for 4 hours.

Main Results:

  • The modified assay demonstrated high sensitivity in detecting penicillin.
  • Detection limits of approximately 0.004 units/ml were achieved with stored plates.
  • As low as 0.002 units/ml penicillin could be detected using freshly prepared materials.

Conclusions:

  • The modified disc assay is a sensitive and rapid method for penicillin detection in milk.
  • This optimized assay can effectively ensure the safety and quality of milk supplies.
  • The method provides a valuable tool for regulatory agencies and the dairy industry.