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Published on: December 16, 2015
A Practical Sensitive Test to Detect Penicillin in Milk
1Cincinnati Training Facility, Division of Federal-State Relations EDRO, Food and Drug Administration, Room 8002 Federal Building 550 Main Street, Cincinnati, Ohio 45202.
Abstract:
The disc assay of the International Dairy Federation for determination of penicillin in milk has been modified in the following steps: preparation of seed broth, seeded plates, and standard solutions. The modified assay is sensitive and relatively rapid, requiring a 4-h incubation at 55 C. Approximately 0.004 unit of penicillin/ml of milk can be detected on plates stored for 7 days; with freshly prepared plates and standard solutions, as little as 0.002 unit of penicillin/ml can be detected.
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