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Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
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Estimating Somatic Cells in Milk Samples by the Membrane-Filter-DNA Procedure.

Robert D Bremel1, L H Schultz1, F Raymond Gabler1

  • 1Department of Dairy Science, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706; and Millipore Corporation, Bedford, Massachusetts 01730.

Journal of Food Protection
|February 9, 2019
PubMed
Summary
This summary is machine-generated.

The membrane-filter DNA test offers a more precise and unbiased method for determining milk

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Area of Science:

  • Dairy Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Somatic cell count (SCC) is a critical indicator of milk quality and udder health.
  • Current methods like direct microscopic somatic cell count (DMSCC) have limitations in precision and potential for technician bias.
  • Advancements in DNA-based methods offer potential for improved accuracy in SCC determination.

Purpose of the Study:

  • To describe further developments in the membrane-filter DNA procedure for milk somatic cell content analysis.
  • To statistically compare the membrane-filter DNA test with the direct microscopic somatic cell count.
  • To establish a standardization method for the membrane-filter procedure.

Main Methods:

  • Development and refinement of the membrane-filter DNA assay for milk samples.
  • Statistical comparison between the membrane-filter DNA test and direct microscopic somatic cell count.
  • Utilization of lyophilized bovine somatic cells on membrane filters for standardization.

Main Results:

  • A quantitative relationship was established: 0.093 absorbance units per million cells at 490 nm.
  • The membrane-filter DNA technique demonstrated higher precision compared to the direct microscopic count.
  • The membrane-filter method is not susceptible to technician bias, ensuring greater reliability.

Conclusions:

  • The developed membrane-filter DNA procedure provides a more accurate and reliable method for somatic cell content determination in milk.
  • Standardization using lyophilized bovine somatic cells ensures consistent and comparable results across different laboratories.
  • This improved method contributes to better milk quality assessment and dairy herd management.