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Published on: November 26, 2010
Collaborative Study of the Coulter Counter-Chemical Method for Counting Somatic Cells in Raw Milk 1
R E Ginn1, D R Thompson1, V S Packard1
1Dairy Quality Control Institute, Inc., St. Paul, Minnesota 55113, and Department of Agricultural Engineering and Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55108.
Abstract:
Variation between laboratories for Electronic Somatic Cell Counting by the chemical method (ESCC) was evaluated by a collaborative study. Eight laboratories counted somatic cells in 12 milk samples (six replicated samples) by the ESCC method. The somatic cell count for the same milk samples was also evaluated by the Direct Microscopic Somatic Cell Counting procedure (DMSCC) as a comparison for the level of error. The standard deviation of the variation of logarithms of ESCC counts between laboratories was 0.04368. The standard deviation for the variation of logarithms of DMSCC counts between technicians was 0.08617. The corresponding value for the DMSCC analysis of the last set of federal split milk samples was 0.141. An earlier study of electronic counting by the centrifuge method showed a standard deviation of 0.0711.
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