Related Experiment Video
Updated: Jan 29, 2026
05:21
A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
Published on: August 14, 2019
26.1K
First Report of Tomato chlorosis virus Infecting Sweet Pepper in Costa Rica
J A Vargas1, E Hernández2, N Barboza2
1Universidad de Costa Rica, CIBCM, San José, Costa Rica; R. Hammond, USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD.
Plant Disease
|February 9, 2019
Summary
Tomato chlorosis virus (ToCV) was detected in sweet pepper plants in Costa Rica, causing chlorosis and leaf curling. This marks the first report of ToCV infecting sweet pepper in the region and the third globally, posing a threat to vegetable production.
Area of Science:
- Plant Pathology
- Virology
- Agricultural Science
Background:
- Whiteflies, specifically Trialeurodes vaporariorum, were observed in high populations on symptomatic sweet pepper plants in Costa Rica.
- Sweet pepper plants (Capsicum annuum cv. Nataly) exhibited symptoms including interveinal chlorosis, enations, necrosis, and upward leaf curling.
Purpose of the Study:
- To investigate the presence of whitefly-borne viruses in symptomatic sweet pepper plants in Cartago, Costa Rica.
- To identify the specific virus causing the observed symptoms in sweet pepper crops.
Main Methods:
- Real-time PCR assays were conducted using three sets of primers to detect Tomato chlorosis virus (ToCV) in 165 symptomatic sweet pepper samples.
- Sequence analysis of PCR products confirmed 100% identity with a known ToCV isolate from the United States.
- Begomovirus PCR detection assays were performed, yielding negative results.
Main Results:
- Seven out of 165 sweet pepper samples tested positive for ToCV through PCR amplification and sequence analysis.
- The identified ToCV isolate showed 100% sequence identity to a US tomato isolate (GenBank Accession No. AY903448).
- No begomoviruses were detected in the symptomatic plants.
Conclusions:
- This study reports the first occurrence of Tomato chlorosis virus (ToCV) infecting sweet pepper plants in Costa Rica.
- The findings indicate ToCV poses a significant threat to vegetable production in the region, as it has also been found infecting tomatoes and various weeds.
- The widespread presence of symptoms, coupled with a low ToCV detection rate, suggests other factors like nutritional deficiencies may also contribute to plant health issues.
Related Concept Videos
00:50What are Viruses?
128.1K
Overview
128.1K
01:24Data Reporting and Recording
5.4K
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.4K
01:25Types of Reports I: Hands-off Report
1.5K
A hand-off report, also known as a change-of-shift report, is a crucial nursing process that ensures the smooth transition of patient care responsibilities between nursing staff.
Following are the key components and categories of hand-off reports:
Purpose and Process:
Following are the key components and categories of hand-off reports:
Purpose and Process:
1.5K
01:21Types of Reports II: Incident or Occurrence Report
1.3K
An Incident or Occurrence Report in a healthcare setting is a crucial document used to record any unexpected occurrence that may or may not have affected a patient, employee, or visitor. Such reports are critical to improving patient safety and include all details leading up to and including the event.
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
1.3K
01:26Types of Reports III: Telephone and Verbal Reports
1.0K
Telephone and Verbal Reports in healthcare settings are two communication methods for conveying therapeutic instructions from healthcare providers to nurses or other healthcare staff.
Here's an overview of each type:
Telephone Orders
Here's an overview of each type:
Telephone Orders
1.0K
02:11Reporter Genes
13.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.2K