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Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
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Population Dynamics Between Fusarium pseudograminearum and Bipolaris sorokiniana in Wheat Stems Using Real-Time qPCR
Ernesto A Moya-Elizondo1, Barry J Jacobsen2, Andrew C Hogg2
1Instituto de Producción y Sanidad Vegetal, Facultad de Ciencias Agrarias Universidad Austral de Chile, Valdivia, Chile.
Plant Disease
|February 9, 2019
Summary
Fusarium pseudograminearum and Bipolaris sorokiniana cause significant wheat losses. High Fusarium inoculum reduced Bipolaris populations, but both pathogens increased in wheat stems from heading to harvest.
Area of Science:
- Plant Pathology
- Agricultural Science
- Mycology
Background:
- Fusarium pseudograminearum and Bipolaris sorokiniana are major wheat pathogens causing Fusarium crown rot and common root rot.
- Understanding their population dynamics in wheat is crucial for disease management.
Purpose of the Study:
- To investigate the population dynamics of Fusarium pseudograminearum and Bipolaris sorokiniana in spring wheat.
- To assess the impact of single and mixed inoculations on wheat growth and yield.
Main Methods:
- Spring wheat 'Hank' was inoculated with single or mixed inocula of F. pseudograminearum and B. sorokiniana.
- Seedling stand, grain yield, and pathogen populations in wheat stems were quantified using real-time qPCR at different growth stages.
Main Results:
- High inoculum rates of F. pseudograminearum reduced B. sorokiniana populations, but not vice versa.
- Both pathogen populations increased in wheat stems from heading to harvest.
- Inoculations increased infection and co-infection rates; high F. pseudograminearum populations correlated with reduced grain yield.
Conclusions:
- Fusarium pseudograminearum can suppress Bipolaris sorokiniana, but co-infection occurs and increases with inoculation.
- Pathogen population dynamics and interactions influence wheat yield loss.
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