Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

65.0K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.0K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.3K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.3K
Reliability and Validity01:29

Reliability and Validity

13.9K
Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.
13.9K
PCR01:32

PCR

237.3K
Overview
237.3K
Distribution Reliability and Automation01:25

Distribution Reliability and Automation

507
Distribution reliability in electrical power systems is critical for ensuring an uninterrupted power supply to consumers at minimal cost. According to IEEE Standard Terms, reliability is the probability that a device will function without failure over a specified time period or amount of usage. For electric power distribution, this translates to maintaining continuous power supply and addressing customer concerns over power outages. Several indices, as defined by IEEE Standard 1366-2012, are...
507
Specific Heat01:16

Specific Heat

67.4K
The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
67.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The clinical efficacy of unilateral biportal endoscopy contralateral inclinatory approach in the treatment of lumbar foraminal stenosis].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[Nasal infection with Mycobacterium avium complex: a case report].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

Effect of stump-preserving arthroscopic reconstruction or stump-eliminating arthroscopic reconstruction combined with exercise rehabilitation therapy on knee functional recovery in patients with anterior cruciate ligament injuries.

Annals of the Royal College of Surgeons of England·2025
Same author

[A case of thoracic aortic aneurysm after coronary artery bypass grafting was treated with integrative branch stent combined with extracorporeal fenestration technique and internal branch technique].

Zhonghua xin xue guan bing za zhi·2025
Same author

Quantum Electrodynamics in Strong Electromagnetic Fields: Substate Resolved Kα Transition Energies in Heliumlike Uranium.

Physical review letters·2025
Same author

Association of treatment methods and survival in esophageal neuroendocrine carcinoma population of Surveillance, Epidemiology, and End Results database: a retrospective cohort study.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2025

Related Experiment Video

Updated: Jan 29, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.8K

Specific and Sensitive Detection of Enterobacter mori Using Reliable RT-PCR.

M M Lou1, G L Jin2, W X Tian3

  • 1State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

Plant Disease
|February 9, 2019
PubMed
Summary
This summary is machine-generated.

A new polymerase chain reaction (PCR) assay effectively detects Enterobacter mori, the cause of bacterial wilt in mulberry plants. This rapid and specific method is crucial for identifying latent infections and managing this serious mulberry disease.

More Related Videos

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
10:55

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Published on: November 10, 2018

27.9K
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
06:25

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Published on: July 10, 2019

22.5K

Related Experiment Videos

Last Updated: Jan 29, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.8K
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
10:55

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Published on: November 10, 2018

27.9K
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
06:25

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Published on: July 10, 2019

22.5K

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Agricultural Science

Background:

  • Bacterial wilt, caused by Enterobacter mori, poses a significant threat to mulberry production in China.
  • Current control strategies for Enterobacter mori are limited, necessitating effective disease detection methods.
  • Latent infections of Enterobacter mori in mulberry plants complicate disease management.

Purpose of the Study:

  • To develop a rapid and reliable polymerase chain reaction (PCR) assay for the detection of Enterobacter mori.
  • To enable the screening of mulberry plant material for both symptomatic and latent infections of Enterobacter mori.

Main Methods:

  • Primers were designed targeting the RNA polymerase β-subunit (rpoB) gene of Enterobacter mori.
  • The developed PCR assay was validated using various bacterial strains, including Enterobacter type strains and other mulberry pathogens.
  • Real-time PCR was employed to determine the sensitivity of the assay for detecting bacterial DNA and cells in plant tissues.

Main Results:

  • The PCR assay demonstrated 100% sensitivity and 100% specificity against a range of bacterial pathogens and isolates.
  • The assay successfully detected Enterobacter mori DNA at concentrations as low as 10 fg/μl and bacterial cells at 10^2 CFU/ml.
  • Testing of symptomatic plants yielded 100% positive results, while 36.4% of asymptomatic plants showed latent infections.

Conclusions:

  • The developed PCR assay is a fast, simple, reliable, and sensitive tool for detecting Enterobacter mori.
  • This method is suitable for screening mulberry plant material, including asymptomatic plants, for latent infections.
  • The assay will aid in managing bacterial wilt disease in mulberry orchards by enabling early and accurate diagnosis.