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Updated: Jan 29, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Published on: September 27, 2012
Expression of Circadian Clock Genes in Human Colorectal Cancer Tissues Using Droplet Digital PCR
Tugba Orhan1, Peter Böhm Nielsen2, Thomas Vauvert F Hviid2
1a Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen N , Denmark.
Abstract:
Increasing evidence indicates that disruption of circadian rhythms may be directly linked to cancer. Here we report that the expression levels of the core clock genes Per1 and Per3 measured by droplet digital polymerase chain reaction are significantly decreased in tumour tissue from 16 patients undergoing colorectal cancer surgery compared to paired normal mucosa. No differences were observed in the expression of Per2, Bmal1, and Clock. In conclusion, abnormal expression levels of the clock genes Per1 and Per3 in CRC tissue may be related to tumourigenesis and may provide future diagnostic and prognostic information.
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