Ultraclean paired sampling for metal analysis in neurodegenerative disorders

The causes of neurodegenerative disorders are largely unknown. Environmental factors seem to contribute to neurodegeneration in genetically susceptible individuals. Increasing evidence point towards a key role for environmental exposure in the causation of neurodegenerative disorders and specifically for metal exposure. Alterations of metalloproteins seem to be a common motif in neurodegeneration and enough evidence has now accumulated to designate these disorders as metallopathies. Paired sampling refers to the simultaneous sampling of CSF and blood and by comparing metal concentrations between CSF and blood conclusions about exposure and barrier properties can be drawn. However previous reports on metal concentrations in body fluids in neurodegenerative disorders show a wide variation in results hampering firm conclusions on the role of metals in degeneration of nerve cells. Here we suggest some steps and measures to minimise this variation, most important sampling performed in a cleanroom with filtered air and the use of acid washed perfluoroalkoxy vials. By strict adherence to ultraclean paired sampling technique conclusive results concerning the role of metals in neurodegenerative disorders can be generated.