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Gas chromatography-Mass Spectra analysis and deleterious potential of fungal based polythene-degradation products

Manisha K Sangale1,2, Mohd Shahnawaz3,4, Avinash B Ade5

  • 1Department of Botany, Savitribai Phule Pune University, Pune, 411007, Maharashtra, India.

Scientific Reports
|February 9, 2019
PubMed

Insights

This study analyzed polythene-degradation products (PE-DPs) from Aspergillus fungi. PE-DPs showed no negative impact on sorghum seed germination but slightly inhibited seedling elongation, with no harm to tiger sharks.

Area of Science:

  • Environmental microbiology
  • Bioremediation of plastics
  • Fungal degradation of polymers

Background:

  • Polythene, a persistent pollutant, requires effective degradation strategies.
  • Fungal isolates Aspergillus terreus and Aspergillus sydowii show promise in breaking down polythene.
  • Understanding the byproducts of polythene degradation is crucial for assessing environmental safety.

Purpose of the Study:

  • To analyze polythene-degradation products (PE-DPs) generated by efficient fungal isolates.
  • To evaluate the ecotoxicity of PE-DPs on plant (Sorghum) and animal (Tiger shark) systems.
  • To determine the safety of using these fungi for polythene bioremediation.

Main Methods:

  • Fungal isolates (Aspergillus terreus, Aspergillus sydowii) incubated with polythene for 60 days.
  • Polythene-degradation products analyzed using Gas Chromatography-Mass Spectrometry (GC-MS).
  • Ecotoxicity assessed via sorghum seed germination and elongation tests, and tiger shark mortality tests.

Main Results:

  • 24 polythene-degradation products identified in treated samples.
  • Sorghum seed germination rate unaffected; germination index maximized at 50% PE-DP concentration.
  • Highest elongation inhibition rate (34.75%) observed with Aspergillus terreus PE-DPs; no tiger shark mortality recorded.

Conclusions:

  • The analyzed PE-DPs from Aspergillus terreus and Aspergillus sydowii exhibit low ecotoxicity.
  • These fungal isolates are potential candidates for polythene bioremediation with minimal environmental risk.
  • Further research into specific PE-DPs and their long-term effects is warranted.

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