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Updated: Jan 29, 2026

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
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Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

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In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing

Ali M Fouad1, Mahmoud M Gabr1, Elsayed K Abdelhady2

  • 1Department of Biotechnology, Urology and Nephrology Center, Mansoura 35516, Egypt.

Journal, Genetic Engineering & Biotechnology
|February 9, 2019
PubMed

Insights

Muse cells, a rare subpopulation within mesenchymal stem cells (MSCs), demonstrate superior potential for generating insulin-producing cells (IPCs). This study highlights Muse cells as a promising source for functional IPCs.

Area of Science:

  • Stem cell biology
  • Cellular differentiation
  • Endocrinology

Background:

  • Mesenchymal stem cells (MSCs) are a heterogeneous cell population.
  • Muse cells represent a rare, pluripotent subpopulation within MSCs.
  • Understanding Muse cell characteristics is crucial for regenerative medicine.

Purpose of the Study:

  • To evaluate the pluripotency of Muse cells.
  • To compare the ability of Muse cells versus non-Muse cells in generating insulin-producing cells (IPCs) after in vitro differentiation.
  • To assess the functionality of generated IPCs.

Main Methods:

  • Isolation of Muse cells from adipose-derived MSCs using FACSAria III cell sorter.
  • Evaluation of pluripotency markers and stage-specific embryonic antigen-3 (SSEA-3) expression.
  • In vitro differentiation protocol followed by flow cytometry and confocal microscopy to assess IPC generation and insulin production.

Main Results:

  • Muse cells constituted approximately 3.2% of MSCs, with 82% expressing SSEA-3.
  • Pluripotent markers were significantly higher in Muse cells compared to non-Muse cells.
  • A higher percentage of functional IPCs, expressing insulin and C-peptide, were generated from Muse cells.

Conclusions:

  • Muse cells are identified as a distinct adult pluripotent subpopulation.
  • Muse cells possess a superior capacity for generating functional insulin-producing cells compared to non-Muse cells.
  • These findings suggest Muse cells hold significant potential for cell-based therapies in diabetes treatment.
Keywords:
Adipose derived mesenchymal stem cellsFluorescence activated cell sortingMesenchymal stem cellsMuse cellsReal time-quantitative PCR

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