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Updated: Jan 29, 2026

A Minimally Invasive Method for Generating a Syngeneic Orthotopic Mouse Model of Lung Cancer
Published on: August 19, 2025
LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer
Ansheng Wang1, Chengling Zhao2, Yuan Gao3
1Departments of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, No.287 Changhuai Road, Bengbu City, Anhui Province, 233004, People's Republic of China.
Abstract:
Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulators in tumor development. This study aims to investigate the potential role oflncRNALEF1-AS1, in the progression of lung cancer. Quantitative real-time PCR (qRT-PCR) and western blot assays showed that LEF1-AS1 was upregulated while miR-544a was downregulated in lung cancer specimens and cells. Overexpression of LEF1-AS1 led to the enhancement of cell proliferation and invasion, revealed by CCK-8 assay and transwell assay. A negative correlation was found between LEF1-AS1 and miR-544a. BLAST analysis and dual-luciferase assay confirmed that FOXP1 is a downstream effector of miR-544a. Therefore, the LEF1-AS1/miR-544a/FOXP1 axis is an important contributor to lung cancer progression. Collectively, our novel data uncovers a new mechanism that governs tumor progression in lung cancer and provides new targets that may be used for disease monitoring and therapeutic intervention of lung cancer.
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