Related Experiment Video

Updated: Jan 29, 2026

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
23:06

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

Published on: August 11, 2008

19.6K

Matryoshka-type gastro-resistant microparticles for the oral treatment of Mycobacterium tuberculosis

Vanesa Andreu1, Ane Larrea1,2, Pablo Rodriguez-Fernandez3,4,5,6

  • 1Department of Chemical Engineering. Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/Poeta Mariano Esquillor S/N, Zaragoza 50018, Spain.

Nanomedicine (London, England)
|February 9, 2019
PubMed
Abstract

Insights

New Matryoshka-type microparticles protect rifampicin from stomach acid and effectively eliminate Mycobacterium tuberculosis in vitro. These antibiotic nanoparticles offer sustained release and improved efficacy against tuberculosis.

Area of Science:

  • Pharmaceutical Sciences
  • Biotechnology
  • Materials Science

Background:

  • Tuberculosis (TB) remains a significant global health challenge, necessitating novel drug delivery systems.
  • Current treatments for TB can be limited by drug degradation and suboptimal release kinetics.
  • Poly lactic-co-glycolic acid (PLGA) nanoparticles (NP) offer a promising platform for drug delivery, but require protection from harsh physiological conditions.

Purpose of the Study:

  • To develop Matryoshka-type gastroresistant microparticles encapsulating antibiotic-loaded PLGA nanoparticles.
  • To enhance the stability and efficacy of rifampicin against Mycobacterium tuberculosis.
  • To investigate the drug release profile and intestinal barrier penetration of the novel microparticle system.

Main Methods:

  • Synthesis of PLGA nanoparticles loaded with the antibiotic rifampicin using emulsification and evaporation techniques.
  • Development of a methacrylic acid-ethyl acrylate-based coating for gastroresistance.
  • Evaluation of NP stability under simulated gastric conditions and drug release under simulated intestinal conditions.
  • Assessment of antitubercular activity against Mycobacterium tuberculosis in infected macrophages and in vitro intestinal barrier models.

Main Results:

  • The microparticle coating successfully protected rifampicin-loaded PLGA NPs from degradation in simulated gastric conditions.
  • Sustained release of rifampicin was achieved under simulated intestinal conditions.
  • Encapsulation did not compromise the antitubercular activity of rifampicin.
  • The developed NPs demonstrated superior efficacy in eliminating M. tuberculosis compared to free rifampicin in infected macrophages.
  • The antibiotic-loaded PLGA-NPs exhibited the ability to cross an in vitro model of the intestinal barrier.

Conclusions:

  • Matryoshka-type gastroresistant microparticles provide effective protection for antibiotic-loaded PLGA NPs against gastric degradation.
  • The novel system enables sustained antibiotic release and enhanced efficacy against Mycobacterium tuberculosis.
  • These findings suggest a promising new strategy for oral delivery of anti-TB drugs, improving treatment outcomes.
Keywords:
PLGATEERenteric coatingeudragitgastroresistantmicroparticlesoral deliveryrifampicintuberculosis

More Related Videos

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

7.8K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.2K

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.8K
Oral Cavity01:11

Oral Cavity

The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.1K
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
945
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
1.5K
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
611
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.5K