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Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
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Lifetime super-resolution optical fluctuation imaging.

Zhiping Zeng1, Jing Ma1, Xuanze Chen2

  • 1College of Physics and Information Engineering, Fuzhou University, Fuzhou, China.

Journal of Microscopy
|February 9, 2019
PubMed
Summary
This summary is machine-generated.

We introduce lifetime super-resolution optical fluctuation imaging (ltSOFI), a novel super-resolution microscopy technique. This method leverages fluorescence lifetime blinking for enhanced image resolution, demonstrated in simulations and cell imaging.

Keywords:
fluorescence fluctuationlifetime blinkingsuper-resolution

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Super-resolution Imaging

Background:

  • Super-resolution fluorescence microscopy typically relies on modulating fluorescence intensity.
  • Few studies have explored fluorescence lifetime fluctuations for super-resolution imaging.

Purpose of the Study:

  • To propose and demonstrate a new super-resolution imaging scheme in the fluorescence lifetime domain.
  • To explore the potential of fluorescence lifetime blinking for achieving super-resolution.

Main Methods:

  • Development of the lifetime super-resolution optical fluctuation imaging (ltSOFI) scheme.
  • Numerical simulations using high-order cumulant analysis on blinking emitters.
  • Experimental validation using time-lapse FLIM of HeLa cells expressing a cAMP sensor.

Main Results:

  • Proof-of-concept demonstrated through numerical simulations.
  • Successful acquisition of super-resolution lifetime images from cellular samples.
  • Validation of ltSOFI's capability to reconstruct images from fluorescence lifetime blinking.

Conclusions:

  • ltSOFI offers a new dimension in lifetime-based super-resolution microscopy.
  • The technique can enhance spatial resolution in existing fluorescence lifetime imaging and nanoscopy.
  • Provides a new approach for obtaining high-detail fluorescence lifetime images.