Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
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Updated: Jan 29, 2026

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
Published on: January 18, 2019
Sultan Asad1, Fabiana Feitosa-Suntheimer1, Alexander Gold1
1Department of Microbiology, Boston University School of Medicine; National Emerging Infectious Diseases Laborator, Boston University School of Medicine.
Abstract:
The recent emergence of the flavivirus Zika and neurological complications, such as Guillain-Barré syndrome and microcephaly in infants, has brought serious public safety concerns. Among the risk factors, antibody-dependent enhancement (ADE) poses the most significant threat, as the recent re-emergence of the Zika virus (ZIKV) is primarily in areas where the population has been exposed and is in a state of pre-immunity to other closely related flaviviruses, especially dengue virus (DENV). Here, we describe a protocol for quantifying the effect of human serum antibodies against DENV on ZIKV infection in primary human cells or cell lines.
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