Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gravimetry: Inorganic And Organic Precipitating Agents00:49

Gravimetry: Inorganic And Organic Precipitating Agents

7.1K
In gravimetry, the precipitant is chosen carefully to obtain a pure solid that can be easily filtered. Common inorganic precipitants can be used to determine several cations and anions. In some cases, the formation of the same precipitate can be used to determine the cation and the anion. For example, the reaction of barium and chromate ions to give barium chromate is used to determine both barium and chromate. However, precipitates such as hydroxides, oxalates, and metal ammonium phosphates...
7.1K
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

523
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
523
The Tree of Life - Bacteria, Archaea, Eukaryotes02:40

The Tree of Life - Bacteria, Archaea, Eukaryotes

38.4K
The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...
38.4K
Other Unique Bacteria01:18

Other Unique Bacteria

436
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
436
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

657
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
657
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

538
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
538

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probiotic biogeography and sepsis prevention in the neonatal intestine.

bioRxiv : the preprint server for biology·2025
Same author

A Defined Artificial Saliva Medium Allows Characterization of the Hypothiocyanite Response of Streptococcus sanguinis and Streptococcus mutans.

Molecular oral microbiology·2025
Same author

Identification of polyphosphate-binding proteins in <i>Escherichia coli</i> uncovers targets involved in translation control and ribosome biogenesis.

mBio·2025
Same author

Genome-wide characterization of hypothiocyanite stress response in <i>Escherichia coli</i>.

Journal of bacteriology·2025
Same author

Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology in <i>Escherichia coli</i>.

mBio·2024
Same author

Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology in <i>Escherichia coli</i>.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Jan 29, 2026

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

9.2K

Assaying for Inorganic Polyphosphate in Bacteria.

Arya Pokhrel1, Jordan C Lingo2, Frank Wolschendorf2

  • 1Department of Microbiology, University of Alabama at Birmingham.

Journal of Visualized Experiments : Jove
|February 9, 2019
PubMed
Summary

This study introduces a streamlined, cost-effective method for quantifying inorganic polyphosphate (polyP) in bacteria. The new technique enables reliable polyP measurement across diverse bacterial species, aiding research into bacterial virulence and stress responses.

More Related Videos

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.5K
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
10:20

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae

Published on: July 10, 2015

16.5K

Related Experiment Videos

Last Updated: Jan 29, 2026

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

9.2K
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.5K
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
10:20

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae

Published on: July 10, 2015

16.5K

Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Inorganic polyphosphate (polyP) is a ubiquitous biological polymer crucial for bacterial virulence and stress response.
  • Existing methods for polyP quantification are often labor-intensive or lack sensitivity, hindering research.
  • A need exists for a more efficient and reliable method for bacterial polyP analysis.

Purpose of the Study:

  • To develop and validate a streamlined, inexpensive, and sensitive method for quantifying inorganic polyphosphate (polyP) in various bacterial species.
  • To provide a protocol for the purification of the polyP-specific enzyme ScPPX, which is essential for the assay but not commercially available.

Main Methods:

  • Utilized a silica membrane column extraction for rapid, high-throughput processing of bacterial samples.
  • Employed the polyP-specific exopolyphosphatase ScPPX for complete digestion of polyP into free phosphate.
  • Quantified released phosphate using a sensitive ascorbic acid-based colorimetric assay.

Main Results:

  • Successfully quantified polyP in diverse bacterial species, including Escherichia coli (Gram-negative), Lactobacillus reuteri (Gram-positive), and Mycobacterium smegmatis (mycobacterial).
  • Demonstrated the method's reliability, cost-effectiveness, and ease of use for multiple samples.
  • Provided a simple protocol for nickel affinity purification of ScPPX enzyme.

Conclusions:

  • The developed method offers a significant improvement for bacterial polyP quantification, overcoming limitations of previous techniques.
  • This assay facilitates further investigation into the roles of polyP in bacterial physiology, pathogenesis, and stress adaptation.
  • The availability of purified ScPPX enhances the accessibility of this quantification method for the research community.