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Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis.

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This study evaluated the Axiom Microbiome Array for detecting diverse microbes in veterinary samples. The comprehensive array demonstrated high sensitivity and specificity for identifying viral and bacterial pathogens in clinical specimens.

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Area of Science:

  • Microbiology
  • Genomics
  • Veterinary Diagnostics

Background:

  • Microarrays offer rapid detection of viral and bacterial pathogens.
  • Existing platforms are used for clinical diagnostics and biological material safety evaluation.
  • Comprehensive microbial detection is crucial for understanding complex ecosystems.

Purpose of the Study:

  • To evaluate the sensitivity, specificity, and utility of the Axiom Microbiome Array for microbiome analysis in veterinary clinical samples.
  • To assess the array's capability in detecting a wide range of microbial species.
  • To determine the array's effectiveness in identifying specific pathogens in co-infected animal samples.

Main Methods:

  • The Axiom Microbiome Array, containing probes for over 12,000 species, was utilized.
  • Sensitivity was tested using known quantities of bacterial and viral DNA (Shigella, Aspergillus, vaccinia virus).
  • Specificity was assessed using mock microbial communities and real veterinary samples (serum, tissue, fecal).

Main Results:

  • The array detected Shigella and Aspergillus at 100 genome copies and vaccinia virus at 1,000 genome copies.
  • Accurate species-level identification was achieved in mock microbial communities.
  • The array correctly identified porcine reproductive and respiratory syndrome virus and porcine circovirus type 2, along with other common microbes, in co-infected pig samples.

Conclusions:

  • The Axiom Microbiome Array is a highly sensitive and specific tool for comprehensive microbiome analysis.
  • This cost-effective, high-throughput platform enables rapid detection of multiple viral and bacterial pathogens in clinical and environmental samples.
  • The array is a valuable asset for veterinary diagnostics and pathogen surveillance.