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Updated: Jan 29, 2026
11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
11.2K
Widespread and Functional RNA Circularization in Localized Prostate Cancer.
Sujun Chen1, Vincent Huang2, Xin Xu3
1Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada; Princess Margaret Cancer Center, University Health Network, Toronto, Ontario, Canada; Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
Cell
|February 9, 2019
Summary
Ultra-deep RNA sequencing of prostate cancer reveals distinct transcriptomic subtypes and widespread circular RNA production. These circular RNAs are essential for cancer cell proliferation and linked to disease progression.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- The cancer transcriptome is complex, containing low-abundance and non-polyadenylated transcripts.
- A comprehensive characterization of localized prostate cancer's transcriptome is needed.
Purpose of the Study:
- To perform ultra-deep total RNA sequencing on localized prostate cancer.
- To identify transcriptomic subtypes and molecular features associated with disease characteristics.
- To investigate the role of circular RNAs in prostate cancer progression and proliferation.
Main Methods:
- Ultra-deep total RNA sequencing (RNA-seq) without poly-A selection on 144 localized prostate tumors.
- Analysis of linear and circular RNA expression, including back-splicing events.
- Loss-of-function screening to assess the essentiality of circular RNAs for cell proliferation.
Main Results:
- Identified a linear transcriptomic subtype associated with aggressive intraductal carcinoma.
- Discovered a fusion profile differentiating localized from metastatic prostate cancer.
- Observed widespread circular RNA (circRNA) production (average 7,232 per tumor), correlated with disease progression.
- Found that 11.3% of abundant circRNAs are essential for cell proliferation, with distinct functions.
Conclusions:
- Ultra-deep RNA-seq without poly-A selection is crucial for comprehensive transcriptome analysis.
- Circular RNAs play significant roles in prostate cancer cell proliferation and progression.
- circRNAs represent potential therapeutic targets and biomarkers for prostate cancer.
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