Related Experiment Video

Updated: Jan 29, 2026

Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs
10:02

Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs

Published on: July 23, 2016

33.4K

Toxicology study of long-term administration of rhKGF-2 eye drops on rabbit corneas

JianQiu Cai1, Wenqing Li2, Jinghang Li3

  • 1Wenzhou Medical University, Chashan University Park, Wenzhou, 325035, China; Department of Ophthalmology, The Affiliated Second Hospital of Wenzhou Medical University, #109 Xueyuan West Road, Wenzhou 325027, China.

Regulatory Toxicology and Pharmacology : RTP
|February 9, 2019
PubMed

Insights

Recombinant human keratinocyte growth factor-2 (KGF-2) eye drops caused temporary ocular irritation in rabbits at high doses. However, KGF-2 eye drops demonstrated no significant corneal toxicity or systemic effects, suggesting a favorable safety profile for corneal repair.

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Toxicology

Background:

  • Keratinocyte growth factor-2 (KGF-2) is known to promote corneal repair by regulating cell regeneration, migration, and stem cell activation.
  • Potential carcinogenic properties and adverse effects of KGF-2 in high-dose, long-term ocular treatments remain largely unreported.

Purpose of the Study:

  • To investigate the potential toxic effects of ocular administration of recombinant human KGF-2 eye drops in a rabbit model.
  • To assess the safety profile of KGF-2 eye drops, particularly concerning corneal health and systemic toxicity.

Main Methods:

  • New Zealand white rabbits were administered varying doses of recombinant human KGF-2 eye drops.
  • Ocular examinations, serum biochemistry analyses, and pathological examinations were conducted to evaluate treatment effects.

Main Results:

  • Animals in medium- and high-dose groups exhibited transient ocular irritant reactions during administration.
  • These irritant reactions were deemed harmless to the cornea and resolved upon cessation of drug administration.
  • Serum biochemistries and pathological examinations were largely unremarkable, indicating no significant systemic toxicity or structural damage.

Conclusions:

  • Over-dosed ocular administration of KGF-2 eye drops can induce temporary, harmless ocular irritation in rabbits.
  • The study suggests that KGF-2 eye drops do not cause significant corneal damage or systemic adverse effects, supporting its potential therapeutic use.
  • Further research may be warranted to fully elucidate the long-term safety and efficacy of KGF-2 in corneal repair therapies.
Keywords:
Corneal injuryKeratinocyte growth factor-2Ocular irritationToxic effects

More Related Videos

Author Spotlight: Optimizing Retinal Tissue Processing for Immunohistochemistry in Rabbit Models05:59

Author Spotlight: Optimizing Retinal Tissue Processing for Immunohistochemistry in Rabbit Models

Published on: November 10, 2023

2.7K
A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study06:13

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study

Published on: August 18, 2011

16.4K

Related Concept Videos

Long-term Depression01:05

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
33.3K
Long-term Depression01:03

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over...
3.4K
Excess Pressure Inside a Drop and a Bubble01:13

Excess Pressure Inside a Drop and a Bubble

The shape of a small drop of liquid can be considered spherical, neglecting the effect of gravity. This drop can further be considered as two equal hemispherical drops put together due to surface tension. The forces acting on the spherical drop are due to the pressure of the liquid inside the drop, the pressure due to air outside the drop, and the force due to the surface tension acting on the two hemispherical drops.
3.4K
Regional Terms01:12

Regional Terms

Regional terms describe anatomy by dividing the body parts into different regions that contain structures involved in contributing similar functions. Using these terms helps increase the accurate description and identification of the particular region of interest or region affected by the disease.
Primarily, the human body has two major regions, the axial and appendicular regions. The axial region comprises regions from the head to the abdomen and makes up the central body axis. In contrast,...
15.4K
Long-Term Memory01:18

Long-Term Memory

Long-term memory is a relatively permanent type of memory, capable of storing vast amounts of information over extended periods. Its storage capacity is generally considered unlimited.
Long-term memory can be categorized into two primary types: explicit and implicit memory. Explicit memory, also known as declarative memory, involves the conscious recollection of information that we deliberately try to remember, recall, and articulate. This type of memory encompasses specific facts, events, and...
673
Long-term Potentiation01:35

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
58.6K