Non-invasive in vivo quantification of human skin tension lines

Human skin is a composite tissue that exhibits anisotropic mechanical properties. This anisotropy arises primarily from the alignment of collagen and elastin fibers in the dermis, which causes the skin to exhibit greater tension in one direction, making it appear stiffer. A diverse number of skin tension guidelines have been developed to assist surgeons in making incisions that produce the least conspicuous scars. However, skin anisotropy is believed to vary from subject to subject, and no single guideline is universally recognized as the best to implement for surgical applications. To date, no system exists that can rapidly and non-invasively measure lines of skin tension in vivo. In this article, we evaluate the ability of a new aspiration system to measure the anisotropy of human skin. The device painlessly applies a radial stress of 17 kPa to a region of skin, and captures radially asymmetric skin deformations via a dermal camera. These deformations are used to quantify orientations of strain extrema and the direction of greatest skin stiffness. The ratio of these asymmetric strains varies between 1 and -0.75. A simple 2D transverse isotropic model captures this behavior for multiple anatomical sites. Clinical trials reveal that skin tension line orientations are comparable with existing skin tension maps and generally agree across subjects, however orientations statistically differ between individuals. As such, existing guidelines appear to provide only approximate estimates of skin tension orientation. STATEMENT OF SIGNIFICANCE: Skin tension lines (STL) in human skin arise primarily from collagen fiber alignment in the dermis. These lines are used by surgeons to guide incisions that produce the least conspicuous scars. While numerous anatomical STL maps exist, no single guideline is universally recognized as the most reliable. Moreover, manual methods of quantifying STL are imprecise. For the first time, we have developed a device capable of rapidly and non-invasively measuring STL orientations in vivo, using a single test. Our results are used to establish a simple constitutive model of mechanical skin anisotropy. Clinical trials further reveal STL orientations are comparable with existing maps, but statistically differ between individuals. Existing guidelines therefore appear to provide only approximate estimates of STL orientation.