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Comparison of Three Assays for Peroxidase in the Blue Crab ( Callinectes sapidus )
Florence S Burnette1, George J Flick1
1Department of Food Science and Technology Seafood Processing Research and Extension Unit Virginia Polytechnic Institute and State University P.O. Box 369, Hampton, Virginia 23669.
Abstract:
Three different enzyme assays which have been used to determine peroxidase activity in food products were compared for their relative sensitivity to measure peroxidase in the blue crab ( Callinectes sapidus ). These included a modified Nagle and Haard, a Nickel and Cunningham, and a Lundquist and Josefsson procedure. The enzyme extracts were tested using a spectrophotometer to measure the ΔOD/min. The modified Nale and Haard procedure was the most sensitive method for detecting peroxidase activity in the blue crab. The assay, which is rapid, can be used with crude extracts and does not require enzyme purification.
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