Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesh Analysis with Current Sources01:10

Mesh Analysis with Current Sources

2.0K
Mesh analysis becomes simpler when analyzing circuits with current sources, whether independent or dependent. The presence of current sources reduces the number of equations required for analysis. Two cases illustrate this:
Current Source in One Mesh: The analysis process is straightforward when a current source is found in only one mesh within the circuit. Mesh currents are assigned as usual, with the mesh containing the current source excluded from the analysis. Kirchhoff's voltage law...
2.0K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.8K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.8K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.7K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.7K
Electrical Current01:10

Electrical Current

7.1K
Electrical current is defined as the rate at which charge flows. When there is a large current present, such as that used to run a refrigerator, a large amount of charge moves through the wire in a small amount of time. If the current is small, such as that used to operate a handheld calculator, a small amount of charge moves through the circuit over a long period of time. The SI unit for current is the ampere (A), named for the French physicist André-Marie Ampère (1775–1836).
7.1K
Sustainable Development01:43

Sustainable Development

15.1K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
15.1K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.5K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complex MINOCA With Myocardial Bridge, Vasospasm, and Microvascular Dysfunction in an HCM Patient.

JACC. Case reports·2026
Same author

Urea Disrupts Lithium Solvation Shells: Multinuclear NMR and MD Insights into Aqueous Electrolytes Based on Deep Eutectic Solvents.

The journal of physical chemistry. B·2026
Same author

Angioleiomyoma Extending From the Iliac Vein to the Right Atrium.

JACC. Case reports·2026
Same author

Transapical Retrieval of an Embolized Valve During Transcatheter Mitral Valve Replacement Using a Retrieval Basket.

JACC. Case reports·2026
Same author

A Very Late Mitral Valve Hypoattenuated Leaflet Thickening With Significant Warfarin Response.

JACC. Case reports·2026
Same author

What problem do you hope bioengineering or synthetic biology approaches will enable us to tackle in the next decade?

Cell systems·2026
Same journal

Lactate Metabolism Dysregulation Drives the Pathogenesis of Acute Kidney Injury.

Metabolites·2026
Same journal

Librarian: An Open-Access Web Application for High-Resolution Mass Spectral Library Assembly.

Metabolites·2026
Same journal

Purine Metabolism Alterations in Patients with Chronic Heart Failure: A Cross-Sectional Study of Associations with Iron Status, Oxidative Stress, and Anemia.

Metabolites·2026
Same journal

The Gut Microbiome in Heart Failure: Pathways to Inflammation and Therapeutic Targets.

Metabolites·2026
Same journal

Metabolic Mechanisms of Hexavalent Chromium-Induced Splenic Immune Injury via Oxidative Stress and Ferroptosis Pathways in New Zealand Rabbits.

Metabolites·2026
Same journal

Improving Speed and Efficiency of DESI Imaging with the Xevo MRT Mass Spectrometer for Analyte Mapping.

Metabolites·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.3K

Current Developments in µMAS NMR Analysis for Metabolomics.

Covadonga Lucas-Torres1, Alan Wong2

  • 1NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette, France. covadonga.lucas-torres-perez@cea.fr.

Metabolites
|February 10, 2019
PubMed
Summary
This summary is machine-generated.

Microscopic Nuclear Magnetic Resonance (NMR) spectroscopy using micro-magic-angle spinning (µMAS) probes enables detailed metabolic profiling of small samples. This technology has advanced significantly, offering new possibilities for metabolomics research.

Keywords:
HR-MASNMRmagic-angle spinningmetabolic profilingmetabolomicsmicro-MAS

More Related Videos

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
11:39

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Published on: June 9, 2019

9.7K
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

6.3K

Related Experiment Videos

Last Updated: Jan 29, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.3K
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
11:39

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Published on: June 9, 2019

9.7K
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

6.3K

Area of Science:

  • Analytical Chemistry
  • Metabolomics
  • Nuclear Magnetic Resonance (NMR) Spectroscopy

Background:

  • Microscopic specimen analysis is crucial for understanding fundamental metabolisms in homogenous samples.
  • Micro-probe technology is established for biofluid NMR but challenging for tissues, cells, and organisms.
  • Developing micro-magic-angle spinning (µMAS) probes for sub-milligram specimens has been a significant hurdle.

Purpose of the Study:

  • To review the development of µMAS probes from inception to current capabilities.
  • To highlight advancements in µMAS technology for microscopic NMR analysis.
  • To discuss the application and potential of µMAS in metabolomics.

Main Methods:

  • Review of µMAS probe development and technological advancements since 2012.
  • Analysis of demonstrative metabolic profiling studies utilizing µMAS NMR.
  • Discussion of challenges and future potential of µMAS in NMR spectroscopy.

Main Results:

  • µMAS probes have evolved from an improbable concept to a viable solution for micro-scale NMR analysis.
  • Demonstrative studies showcase the effectiveness of µMAS for metabolic profiling of small specimens.
  • Continuous advancements have been made, improving data acquisition quality for µg analysis.

Conclusions:

  • µMAS NMR spectroscopy is becoming an effective tool for analyzing microscopic specimens.
  • The technology holds significant potential to advance the field of metabolomics.
  • Overcoming current challenges will further enhance the utility of µMAS in scientific research.