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Micropropagation of Elaeocarpus robustus Roxb.
S K Roy1, M S Islam1, S Hadiuzzaman1
1Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh Fax: 880-2-833054 e-mail: ju@bangla.net, , , , , , BD.
Plant Cell Reports
|February 10, 2019
Summary
This study optimized micropropagation for Elaeocarpus robustus, achieving rapid shoot multiplication and successful rooting. Field survival rates indicate the effectiveness of this plant propagation method.
Area of Science:
- Plant Biotechnology
- Horticultural Science
- Forestry
Background:
- Elaeocarpus robustus is a valuable timber and medicinal tree species.
- Efficient propagation methods are crucial for conserving and utilizing this species.
Purpose of the Study:
- To develop an efficient in vitro propagation protocol for Elaeocarpus robustus.
- To optimize shoot multiplication and rooting for large-scale plant production.
Main Methods:
- Shoot tips and nodal explants were cultured on Murashige and Skoog's medium with BAP and Kinetin.
- Repeated subculturing and supplementation with coconut milk and casein hydrolysate were employed for shoot proliferation.
- Rooting was induced using indole-3-butyric acid and indole-3-acetic acid.
Main Results:
- Multiple shoots were successfully induced from explants.
- Shoot multiplication rates increased significantly with repeated subculturing and additives.
- High rooting efficiency was achieved in vitro.
- A high survival rate (85%) was observed for transplanted plantlets.
Conclusions:
- The established protocol enables efficient mass propagation of Elaeocarpus robustus.
- This method is suitable for the commercial production of Elaeocarpus robustus plantlets.
- The findings contribute to the conservation and sustainable utilization of this important tree species.
