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Updated: Jan 29, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Published on: March 17, 2023
Transformation of arctic bramble (Rubus arcticus L.) by Agrobacterium tumefaciens
1Department of Biochemistry and Biotechnology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland Fax: +358-71-2811510 e-mail: Sirpa.Karenlampi@uku.fi, , , , , , FI.
Abstract:
Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is expressed only in plants. Regenerants were produced on Murashige and Skoog medium. Growth of Agrobacterium was inhibited with cefotaxime. Kanamycin was used as the selective agent for the transformants. Regenerants were assayed by histochemical GUS staining, and by Southern analysis using a gus-int probe. Transgenic arctic bramble plants containing gus-int and expressing GUS were recovered. Expression has been stable for 3 years in micropropagation.
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