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Updated: Jan 29, 2026

A Method for Characterizing Embryogenesis in Arabidopsis
Published on: August 4, 2017
Plant regeneration via somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp)
K Sreenivasu1, S K Malik2, P Ananda Kumar1
1National Research Centre for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India, , , , , , IN.
Abstract:
Efficient plant regeneration via somatic embryogenesis has been developed in pigeonpea. Cotyledon and leaf explants from 10-day-old seedlings produced embryogenic callus and somatic embryos when cultured on Murashige and Skoog (MS) medium supplemented with 10 µM thidiazuron (TDZ). Subsequent withdrawal of TDZ from the induction medium resulted in the maturation and growth of the embryos into plantlets on MS basal medium. The rooted plantlets were transferred and acclimatized on vermiculite where they showed normal morphological characters.
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