Callus induction and somatic embryogenesis of Phalaenopsis
Y Ishii1, T Takamura1, M Goi1
1Department of Horticulture, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan, , , , , , JP.
Abstract:
Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l-1. Medium containing 200 ml ⋅ l-1 coconut water together with 40 g ⋅ l-1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
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