Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.1K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.1K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

10.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
10.1K
Flow Sheet01:17

Flow Sheet

2.7K
Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:
2.7K
Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.6K
Light as Energy01:35

Light as Energy

95.9K
The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
95.9K
The Wave Nature of Light02:12

The Wave Nature of Light

61.3K
The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion.
61.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bio-integrated μBots with overtone ultrawideband magnetoelectric antennas for wireless telemetry.

Science advances·2026
Same author

Functional Brain Network Predictors of Abstinence Treatment Outcomes in Methamphetamine Use Disorder.

CNS neuroscience & therapeutics·2026
Same author

Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.

Signal transduction and targeted therapy·2026
Same author

Measuring the Impacts of Urbanicity and Different Exposome Factors on Human Brain through Exposure Network Mapping.

Neuroscience bulletin·2026
Same author

FOR: Point Cloud Outlier Removal Based on Fuzzy Theory and Informativeness and Its Application to 3D Object Detection.

Sensors (Basel, Switzerland)·2026
Same author

Speckle-based measurement of the fractional azimuthal index of orbital angular momentum beams for refractive index sensing.

Nature communications·2026
Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026
Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026
Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026
Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026
Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026
Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.7K

Light sheet microscopy with acoustic sample confinement.

Zhengyi Yang1,2, Katy L H Cole3, Yongqiang Qiu4,5

  • 1SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK. zhengyiyang.phy@gmail.com.

Nature Communications
|February 10, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a contactless acoustic trap for confining samples in light sheet microscopy, enabling long-term imaging of marine animal development and zebrafish heart function. This method overcomes traditional mounting limitations for advanced biological studies.

More Related Videos

Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction
08:33

Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction

Published on: July 19, 2024

1.4K
Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
05:27

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

2.9K

Related Experiment Videos

Last Updated: Jan 29, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.7K
Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction
08:33

Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction

Published on: July 19, 2024

1.4K
Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
05:27

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

2.9K

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Biophysics
  • Microscopy

Background:

  • Traditional sample mounting in microscopy restricts long-term, wide-field imaging.
  • Contactless methods are needed for advanced developmental biology and neuroscience studies.
  • Light sheet microscopy offers low phototoxicity for live imaging.

Purpose of the Study:

  • To develop and demonstrate a contactless acoustic gradient force trap for sample confinement.
  • To integrate this trap with light sheet microscopy for real-time, environmentally controlled experiments.
  • To showcase the utility of this system for studying marine animal embryos and zebrafish larvae.

Main Methods:

  • Utilized an acoustic gradient force trap for contactless sample confinement.
  • Integrated the trap with light sheet microscopy for wide-field, low phototoxicity imaging.
  • Performed quantitative analysis of zebrafish larval heartbeat dynamics in response to cardiovascular drugs.

Main Results:

  • Successfully demonstrated contactless sample confinement in light sheet microscopy.
  • Enabled long-term, wide-field imaging of marine animal embryos and larvae.
  • Quantified the dynamic cardiovascular response of zebrafish larvae to verapamil and norepinephrine using optical flow analysis.

Conclusions:

  • Contactless acoustic trapping overcomes sample immobilization limitations in microscopy.
  • This technique facilitates real-time drug-based assays and biomechanical analyses in developmental studies.
  • Opens new avenues for research in developmental biology and neuroscience with advanced imaging capabilities.