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Assessing Spatial Learning and Memory in Small Squamate Reptiles
Published on: January 3, 2017
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Reptile Embryology and Regeneration.
Matthew K Vickaryous1, Emily A B Gilbert2,3
1Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada. mvickary@uoguelph.ca.
Methods in Molecular Biology (Clifton, N.J.)
|February 10, 2019
Summary
The leopard gecko is a valuable reptilian model for studying embryonic development and tissue regeneration. This review details husbandry, embryonic development, and advanced histological techniques for research.
Area of Science:
- Developmental biology
- Regenerative medicine
- Herpetology
Background:
- Reptiles are increasingly utilized as models in developmental biology research.
- The leopard gecko (Eublepharis macularius) offers unique advantages for studying embryonic development and regeneration.
- Existing research highlights the need for standardized protocols for gecko research.
Purpose of the Study:
- To present the leopard gecko as a suitable reptilian model for embryonic and tissue regeneration studies.
- To provide comprehensive protocols for husbandry, breeding, and key research techniques.
- To facilitate further research into reptilian developmental biology and regenerative capabilities.
Main Methods:
- Detailed husbandry and breeding protocols for leopard geckos.
- Established protocols for transcardial perfusion, anesthesia (Alfaxan), and cutaneous biopsy.
- Modified histological techniques including whole-mount histochemistry, immunohistochemistry, and double-label immunofluorescence.
- Bromodeoxyuridine (BrdU) labeling and immuno-detection methods.
Main Results:
- The leopard gecko is well-suited for embryonic and regenerative studies.
- Comprehensive protocols for gecko handling, anesthesia, and tissue sampling are provided.
- Optimized histological and molecular techniques are detailed for gecko research.
Conclusions:
- The leopard gecko serves as an excellent model organism for developmental and regenerative research.
- Standardized protocols enhance the reproducibility and scope of studies using this species.
- This review provides a foundational resource for researchers in reptilian developmental biology.
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