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Updated: Jan 29, 2026

Production of Haploid Zebrafish Embryos by In Vitro Fertilization
Published on: July 14, 2014
Generation of Xenopus Haploid, Triploid, and Hybrid Embryos
Romain Gibeaux1, Rebecca Heald2
1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Abstract:
Frog species of the genus Xenopus are widely used for studies of cell and developmental biology, and recent genome sequencing has revealed interesting phylogenetic relationships. Here we describe methods to generate haploid, triploid, and hybrid species starting from eggs and sperm of Xenopus laevis and Xenopus tropicalis that enable investigation of how genome size and content affect physiology at the organismal, cellular, and subcellular levels.
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