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Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).

Sukumal Prukudom1,2,3, Kannika Siripattarapravat1,2,4,3, William Poulos5

  • 1Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, Thailand.

Methods in Molecular Biology (Clifton, N.J.)
|February 10, 2019
PubMed
Summary

Zebrafish are crucial for studying human diseases and development. This protocol details somatic cell nuclear transfer (SCNT) for genetic modification and cell plasticity research in zebrafish.

Keywords:
CloningSomatic cell nuclear transferZebrafish

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Area of Science:

  • Developmental biology
  • Genetics
  • Animal models

Background:

  • Zebrafish (Danio rerio) are widely used in biological research.
  • Existing methods for genetic modification can be limiting.
  • Understanding cell plasticity is key to many biological processes.

Purpose of the Study:

  • To describe a protocol for somatic cell nuclear transfer (SCNT) in zebrafish.
  • To enable genetic modifications in zebrafish.
  • To facilitate the study of cell plasticity.

Main Methods:

  • Somatic cell nuclear transfer (SCNT) procedure.
  • Utilizing zebrafish as the model organism.

Main Results:

  • A detailed protocol for SCNT in zebrafish is presented.
  • The protocol allows for the introduction of genetic modifications.
  • The method is applicable to studying cell plasticity.

Conclusions:

  • SCNT provides a viable method for genetic engineering in zebrafish.
  • This protocol enhances the utility of zebrafish as a model organism.
  • The technique supports research into cell plasticity and disease modeling.