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An immunoliposome-based immunochromatographic strip assay for the rapid detection of Cronobacter species

Xinjie Song1, Shruti Shukla2, Myunghee Kim1

  • 1Department of Food Science and Technology, Yeungnam University, Gyeongsan-si, Gyeongsangbuk-do 38541, Republic of Korea.

Journal of Microbiological Methods
|February 10, 2019
PubMed

Insights

A new immunoliposome assay rapidly detects Cronobacter bacteria, a dangerous pathogen in infant formula. This simple strip test offers effective food safety screening for Cronobacter species.

Area of Science:

  • Food Microbiology
  • Immunodiagnostics
  • Biosensor Technology

Background:

  • Cronobacter species are significant foodborne pathogens, particularly hazardous in infant formula, with high mortality rates in infants.
  • Accurate and rapid detection methods for Cronobacter are crucial for ensuring infant formula safety and preventing outbreaks.
  • Existing detection methods can be time-consuming or require specialized laboratory equipment.

Purpose of the Study:

  • To develop a rapid, simple, and effective immunochromatographic strip assay for detecting Cronobacter species.
  • To specifically target Cronobacter detection in powdered infant formula (PIF).
  • To evaluate the assay's performance in terms of detection limits and accuracy compared to quantitative analysis.

Main Methods:

  • Development of an immunoliposome-based immunochromatographic strip assay using anti-Cronobacter immunoglobulin G (IgG) conjugated liposomes.
  • Utilizing a nitrocellulose membrane coated with anti-Cronobacter IgG.
  • Testing the assay with pure cultures and artificially contaminated PIF samples.
  • Analyzing results via visual judgment and quantitative analysis using QuantiScan software.

Main Results:

  • The assay successfully detected Cronobacter species in pure culture and contaminated PIF.
  • Visual detection limits were 10^6-10^7 CFU/mL (pure culture) and 10^7-10^8 CFU/g (PIF).
  • QuantiScan analysis improved detection limits to 10^5-10^7 CFU/mL (pure culture) and 10^6-10^8 CFU/g (PIF), though Cronobacter malonaticus detection was initially challenging visually but improved with QuantiScan.

Conclusions:

  • The developed immunoliposome-based immunochromatographic strip assay is a simple, rapid, and effective tool for Cronobacter detection.
  • The assay shows potential for widespread application in the food industry, research, and onsite testing.
  • Further optimization may enhance sensitivity for specific species like Cronobacter malonaticus.

Keywords:
Cronobacter speciesFoodborne pathogensImmunochromatographic stripImmunoliposomePowdered infant formula

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