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Updated: Jan 29, 2026

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process
Published on: March 21, 2014
Optimization of preparation conditions for CTAB-modified Polygonatum sibiricum polysaccharide cubosomes using the
Haiyu Ni1, Shuwen Xu1, Pengfei Gu1
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Abstract:
This study aimed to optimize the preparation conditions for cetyltrimethylammonium bromide-modified Polygonatum sibiricum polysaccharide cubosomes (CTAB-modified PSP-Cubs) by response surface methodology (RSM). Glyceryl-monooleate (GMO) was used as the lipid base for the cubosomes. The optimal preparation conditions of CTAB-modified PSP-Cubs were as follows: the mass percentage of PSP to GMO (X1), Poloxamer 407 (F127) to GMO (X2) and water to GMO (X3) was 1.4%, 9% and 50%, respectively. The encapsulation efficiency (EE) of CTAB-modified PSP-Cubs under the experimental conditions was 62.4 ± 3.6%, which was close to our predicted value. The particle size, polydispersity index (PDI) and zeta potential of CTAB-modified PSP-Cubs were 427.7 ± 8.0 nm, 0.236 ± 0.024 and 19.2 ± 0.4 mV, respectively. Specific modifications were able to slow down in vitro release behaviors and reduce cytotoxicity to some extent. The effects of CTAB-modified PSP-Cubs on splenic lymphocytes were also investigated. When splenic lymphocytes were treated with CTAB-modified PSP-Cubs together with LPS or PHA, the results showed more favorable effects on cellular proliferation than blank cubosomes or free PSP at certain concentrations.
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