Injectable chitosan-nano bioglass composite hemostatic hydrogel for effective bleeding control

Area of Science:

• Biomaterials Science
• Biomedical Engineering
• Hemostasis Research

Background:

• Effective bleeding control is critical in trauma and surgery.
• Chitosan hydrogels are used as hemostatic agents but require improvement.
• Nanobioglass incorporation aims to enhance chitosan's clotting properties.

Purpose of the Study:

• To improve the blood clotting efficacy of chitosan hydrogel.
• To develop a chitosan-nanobioglass (Ch-nBG) hydrogel for rapid hemostasis.
• To evaluate the hemostatic and biocompatibility potential of the Ch-nBG hydrogel.

Main Methods:

• Nanobioglass (nBG) particles synthesized via sol-gel method (particle size 14±3 nm).
• 2% Chitosan-5% nanobioglass (2%Ch-5%nBG) hydrogel prepared.
• Characterization using SEM and FTIR; evaluation of injectability and cytocompatibility (HUVEC).
• In vitro blood clotting assays and in vivo major organ injury models.

Main Results:

• The 2%Ch-5%nBG hydrogel demonstrated enhanced rapid blood clot formation compared to 2%Ch hydrogel.
• SEM and FTIR confirmed hydrogel structure and composition.
• The hydrogel was injectable and exhibited cytocompatibility with HUVEC cells.
• Significant improvement in hemostatic efficacy observed in both in vitro and in vivo studies.

Conclusions:

• The developed 2%Ch-5%nBG hydrogel significantly accelerates blood clotting.
• This novel hydrogel shows promising potential as an effective hemostatic agent.
• The Ch-nBG hydrogel could be a valuable tool for bleeding control in critical medical scenarios.