Monitoring photodynamic oxygen consumption by endogenous oxygen contrast MRI

Photodynamic oxygen consumption was measured by changes in spin-lattice relaxation time (T₁) in aqueous solution in a clinical GE scanner at 1.5 T. Similar measurements were attempted in excised laryngeal and thyroid tissues that were infused with Rose Bengal. First, T₁ was measured as a function of dissolved oxygen in argon and in oxygen pre-saturated water samples that were opened to the atmosphere in a series of steps allowing air to diffuse into or out of solution; for both argon and oxygen saturated water solutions, stepwise air re-equilibration resulted in a return to air-saturated water T₁. Secondly, T₁ was measured as a function of time under type II photooxidative conditions in aqueous solution. Under type II photooxidative conditions, a 492 ± 53 ms increase in T₁ was measured following 300 s of visible light illumination of aqueous solutions containing the photosensitizer Rose Bengal (2.5 × 10^-6 M) and the singlet oxygen trap methionine (0.0012 M). The 492 ± 53 ms increase in T₁ corresponded to consumption of all the measurable dissolved oxygen (˜ 0.1 mg O₂ in 15.0 mL of H₂O) during photooxidation of methionine in air saturated water. This rapid oxygen consumption, indicated by an increase in T₁, is due to irreversible trapping of photogenerated singlet oxygen by methionine. Thirdly, an increase in T₁ was observed in Rose Bengal infused normal laryngeal tissue, and in normal and cancerous thyroid tissue samples following 20 min of exposure to visible light. An increase in T₁ was not observed after 40 min of illumination which suggests that the increases in T₁ observed after 20 min were not due to water uptake, but rather to photoconsumption of interstitial dissolved oxygen.