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Insulin modulation of pyruvate dehydrogenase in human circulating lymphocytes
M Curto1, M Piccinini, R Bruno
1Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, Italia.
The International Journal of Biochemistry
|January 1, 1988
Abstract:
1. In human circulating lymphocytes pyruvate dehydrogenase (PDH) complex is present in the active (PDHa) and inactive (PDHi) forms. 2. PDHi conversion into PDHa is stimulated when intact lymphocytes are incubated with 5 microU/ml insulin at pH 7.4, for 15 min at 37 degrees C in a medium supplemented with 50 microM Ca2+-Mg2+. 3. The generation of a mediator is strongly suggestive since a cell free preparation from circulating lymphocytes, treated as above described, still stimulates PDHi----PDHa conversion, when combined with either disrupted or intact lymphocytes.