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Updated: Jan 29, 2026

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research
Published on: July 3, 2009
Behavioural data on instar crab movement at different thermal acclimation
Mohamad N Azra1, Mhd Ikhwanuddin1, Ambok Bolong Abol-Munafi1
1Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, Malaysia.
Abstract:
This article investigated how crabs responded to different culture temperatures especially dislocation before molting using a combination of large recording files and computer software. In this novel approach of video recording portunid crab behavioral data, crab culture was recorded at five different acclimation temperatures of 20, 24, 28, 32 and 36 °C. Crabs were reared until the instar stage before being acclimatized for video recording. Large video files (MPEG-TS) were then analyzed using the latest version of Solomon Coder software developed by A. Peter and programmed with Embarcadero® Delphi® XE [1]. Recorded data was analyzed by calculating and marking movements of crabs using the time sequence tool. Additionally, a total movement was counted 30 min before crabs molted from instar stage 8 to instar stage 9. Part of the data is associated with the research article "Thermal tolerance and locomotor activity of blue swimmer crab Portunus pelagicus instar reared at different temperatures" (Azra et al., 2018) [2] and provided here as raw data of Supplementary materials.
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