MicroRNA Profile Differences in Neonates at Risk for Cerebral Palsy

MicroRNAs; miRs are used as biomarkers in the diagnosis of several diseases. Cerebral palsy; CP, resulting from perinatal brain injury, cannot be diagnosed until 18-24 months old. Biomarkers to predict CP and assess response to investigational therapies are needed. We hypothesized that miRs expressed in neonates with the CP risk factors of abnormal tone and/or intraventricular hemorrhage; IVH differ from those without risk factors.

This was a cohort study of neonates at risk for CP. Subjects <32 weeks gestation and <1500 grams were recruited from neonatal intensive care units at a large urban delivery hospital and an adjacent children's hospital. Thirty-one plasma samples were evaluated. An unbiased examination was performed by locked nucleic acid quantitative real time - polymerase chain reaction; qRT-PCR. Results were evaluated in the context of IVH and abnormal tone.

Plasma miR profiles in neonates at risk for CP differ when comparing those with and without IVH, and with and without abnormal tone. Restricted profiles were found in each condition with greater differences in the tone comparison than the IVH comparison.

Plasma miR profiles show potential in predicting CP. This study also suggests biologically plausible candidates for future studies.