Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mutations01:39

Mutations

94.5K
Overview
94.5K
Mutations01:35

Mutations

44.5K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
44.5K
Viral Mutations00:36

Viral Mutations

39.9K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.9K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.9K
Nuclear Stability03:18

Nuclear Stability

23.2K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
23.2K
RNA Stability01:53

RNA Stability

35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fast sampling of protein conformational dynamics.

Science advances·2026
Same author

Cell Painting reveals polypharmacological activity on the V-ATPase by the F-ATPase inhibitor Cyhexatin.

Pesticide biochemistry and physiology·2026
Same author

Unveiling the entropic role of hydration water in SOD1 partitioning within FUS condensate.

The Journal of chemical physics·2026
Same author

Beyond contacts: The important role of the support region in protein complex assembly.

Protein science : a publication of the Protein Society·2026
Same author

Cytoplasmic fluidity and the cold life: proteome stability is decoupled from viability in psychrophiles.

Nature communications·2025
Same author

Unraveling the Link Between Thermal Adaptation and Latent Allostery in Malate Dehydrogenase From Methanococcales.

Journal of molecular biology·2025

Related Experiment Video

Updated: Jan 29, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

13.2K

Stability Effect of Quinary Interactions Reversed by Single Point Mutations.

David Gnutt1,2, Stepan Timr3, Jonas Ahlers2

  • 1Institute of Physical and Theoretical Chemistry , TU Braunschweig , Rebenring 56 , Braunschweig 38106 , Germany.

Journal of the American Chemical Society
|February 12, 2019
PubMed
Summary

Cellular quinary interactions, or protein-macromolecule interactions, can destabilize proteins like superoxide dismutase I (SOD1). However, altered surface properties in SOD1 mutants can surprisingly stabilize the protein in cells.

More Related Videos

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.3K
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

12.1K

Related Experiment Videos

Last Updated: Jan 29, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

13.2K
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.3K
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

12.1K

Area of Science:

  • Structural biology
  • Biophysics
  • Cellular biology

Background:

  • Proteins function within a crowded cellular environment.
  • Protein-macromolecule interactions (quinary interactions) influence protein stability, function, and localization.
  • Understanding these interactions is crucial for molecular-level insights into cellular processes.

Purpose of the Study:

  • To investigate the energetic contributions of quinary interactions to protein stability.
  • To quantify the impact of single amino acid mutations on superoxide dismutase I (SOD1) stability in mammalian cells.
  • To explore how quinary interactions modulate the effects of mutations on protein behavior.

Main Methods:

  • Utilized mutational analysis to assess individual amino acid contributions.
  • Quantified energetic changes related to protein stability.
  • Compared protein stability in cellular environments versus in vitro conditions.

Main Results:

  • Quinary interactions generally destabilize SOD1, with a consistent energetic offset for most mutants.
  • Mutations altering SOD1 surface properties demonstrated exceptions, leading to protein stabilization within cells compared to test tubes.
  • Quinary interactions can significantly amplify or even reverse the destabilizing effects of mutations.

Conclusions:

  • Quinary interactions play a critical role in modulating protein stability and response to mutations.
  • These interactions can amplify or reverse mutational effects, impacting protein behavior.
  • Understanding quinary interactions is key to comprehending pathogenic protein misfolding and aggregation, particularly in diseases like ALS.