Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Case Studies01:22

Case Studies

13.5K
There are many research methods available to psychologists in their efforts to understand, describe, and explain behavior and the cognitive and biological processes that underlie it.
13.5K
Imaging Studies I: Kidney, Ureter, and Bladder Studies01:28

Imaging Studies I: Kidney, Ureter, and Bladder Studies

344
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
344
Longitudinal Studies01:26

Longitudinal Studies

521
Longitudinal studies are also widely used in other medical and social science fields. For instance, in cardiovascular research, they can monitor patients' health over decades to identify risk factors for heart disease, such as high cholesterol or smoking, and evaluate the long-term effectiveness of preventive measures. Similarly, in mental health studies, researchers might follow individuals from adolescence into adulthood to understand the development and progression of conditions like...
521
Observational Studies01:11

Observational Studies

11.0K
Observational studies are a type of analytical study where researchers observe events without any interventions. In other words, the researcher does not influence the response variable or the experiment's outcome.
There are three types of observational studies – Prospective, retrospective, and cross-sectional.
Prospective Study
Prospective studies, also known as longitudinal or cohort studies, are carried out by collecting future data from groups sharing similar characteristics. One...
11.0K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

9.4K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
9.4K
Bioavailability Study Design: Single Versus Multiple Dose Studies01:11

Bioavailability Study Design: Single Versus Multiple Dose Studies

232
Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...
232

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Route of BCG administration determines success.

Human vaccines & immunotherapeutics·2026
Same author

Newborns with sickle cell trait have distinct peripheral blood molecular signatures across the first week of life.

Blood. Red cells & iron·2026
Same author

Maternal and Newborn Group B <i>Streptococcus</i> Serology in the United States and South Africa.

Open forum infectious diseases·2026
Same author

Nonlinear Modeling Reveals Novel Associations Between Genetically Predicted Protein Levels and Pancreatic Cancer Risk.

Molecular carcinogenesis·2026
Same author

Population-specific heterogeneity in ontogeny of the broadly-conserved blood transcriptional program during the first week of life.

Nature communications·2026
Same author

Rezvilutamide in combination with androgen deprivation therapy (ADT) with or without docetaxel in the neoadjuvant treatment of oligometastatic prostate cancer (REDOOM): study protocol for an open-label, parallel-cohort, multicentre clinical study.

BMJ open·2026

Related Experiment Video

Updated: Jan 29, 2026

Probiotic Studies in Neonatal Mice Using Gavage
10:36

Probiotic Studies in Neonatal Mice Using Gavage

Published on: January 27, 2019

20.7K

Probiotic Studies in Neonatal Mice Using Gavage.

Freddy Francis1, Natallia Varankovich2, Byron Brook3

  • 1Department of Experimental Medicine, University of British Columbia; fredfrancis@me.com.

Journal of Visualized Experiments : Jove
|February 12, 2019
PubMed
Summary

Developing a neonatal mouse model is crucial for studying infant diseases. This study details a standardized intra-esophageal gavage method for administering probiotics like Lactobacillus plantarum (LP) to newborn mice, enabling colonization assessment.

More Related Videos

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
12:12

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Published on: February 8, 2016

13.0K
Intravenous Injections in Neonatal Mice
05:17

Intravenous Injections in Neonatal Mice

Published on: November 11, 2014

61.3K

Related Experiment Videos

Last Updated: Jan 29, 2026

Probiotic Studies in Neonatal Mice Using Gavage
10:36

Probiotic Studies in Neonatal Mice Using Gavage

Published on: January 27, 2019

20.7K
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
12:12

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Published on: February 8, 2016

13.0K
Intravenous Injections in Neonatal Mice
05:17

Intravenous Injections in Neonatal Mice

Published on: November 11, 2014

61.3K

Area of Science:

  • Neonatal immunology
  • Microbiome research
  • Animal models

Background:

  • Adult mouse models are limited for studying neonatal diseases.
  • Neonatal mouse models are essential for understanding infant disease mechanisms, host responses, and intervention impacts.
  • Technical challenges with small animals hinder neonatal mouse model utilization.

Purpose of the Study:

  • To develop a neonatal mouse model for studying early-life interventions.
  • To assess the colonization ability of probiotics in the neonatal mouse intestinal tract.
  • To establish a standardized method for probiotic administration in newborn mice.

Main Methods:

  • Developed a standardized intra-esophageal (IE) gavage technique for precise probiotic delivery to neonatal mice.
  • Administered Lactobacillus plantarum (LP) via IE gavage to assess colonization.
  • Utilized quantitative polymerase chain reaction (qPCR) with LP-specific primers to trace probiotic colonization in the intestinal tract.
  • Investigated colonization spread using different litter settings and cage management.

Main Results:

  • A reproducible IE gavage method was established, minimizing trauma and aspiration risk.
  • Successful colonization of Lactobacillus plantarum (LP) in the neonatal mouse gut was confirmed via qPCR.
  • The study explored factors influencing probiotic colonization spread within litters.

Conclusions:

  • The developed IE gavage technique is an improvement for neonatal mouse studies.
  • This model allows for accurate assessment of probiotic colonization and early-life intervention effects in neonates.
  • Further research can utilize this model to investigate host-microbiome interactions in early life.