Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Control System Problem01:21

Control System Problem

427
In an open-loop system, such as a basic thermostat, the poles of the transfer function influence the system's response but do not determine its stability. However, when feedback is introduced to form a closed-loop system, such as an advanced thermostat that adjusts heating based on room temperature, stability is governed by the new poles of the closed-loop transfer function.
When forming a closed-loop system, issues can arise if the poles cross into the unstable region, leading to potential...
427
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.6K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.6K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.6K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.6K
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

464
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
464
Control Systems01:10

Control Systems

1.9K
Control systems are everywhere in contemporary society, influencing diverse applications from aerospace to automated manufacturing. These systems can be found naturally within biological processes, such as blood sugar regulation and heart rate adjustment in response to stress, as well as in man-made systems like elevators and automated vehicles. A control system is essentially a network of subsystems and processes that collaboratively convert specific inputs into desired outputs.
At the heart...
1.9K
Controller Configurations01:22

Controller Configurations

377
Controller configurations are crucial in a car's cruise control system because they manage speed over time to maintain a consistent pace regardless of road conditions, thereby meeting design goals. In traditional control systems, fixed-configuration design involves predetermined controller placement. System performance modifications are known as compensation.
Control-system compensation involves various configurations, most commonly series or cascade compensation, in which the controller...
377

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Route of BCG administration determines success.

Human vaccines & immunotherapeutics·2026
Same author

Newborns with sickle cell trait have distinct peripheral blood molecular signatures across the first week of life.

Blood. Red cells & iron·2026
Same author

Maternal and Newborn Group B <i>Streptococcus</i> Serology in the United States and South Africa.

Open forum infectious diseases·2026
Same author

Population-specific heterogeneity in ontogeny of the broadly-conserved blood transcriptional program during the first week of life.

Nature communications·2026
Same author

Rezvilutamide in combination with androgen deprivation therapy (ADT) with or without docetaxel in the neoadjuvant treatment of oligometastatic prostate cancer (REDOOM): study protocol for an open-label, parallel-cohort, multicentre clinical study.

BMJ open·2026
Same author

Long-term safety of the Pfizer-BioNTech COVID-19 vaccine: Results from the HERO-together study.

Respiratory medicine·2026

Related Experiment Video

Updated: Jan 29, 2026

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
14:54

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Published on: January 27, 2019

11.8K

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis.

Byron Brook1, Nelly Amenyogbe2, Rym Ben-Othman3

  • 1Department of Experimental Medicine, University of British Columbia; bbrook@bcchr.ubc.ca.

Journal of Visualized Experiments : Jove
|February 12, 2019
PubMed
Summary

This study presents a standardized preclinical model for neonatal sepsis using cecal slurry in mice. The method includes detailed steps for inducing sepsis and defining humane endpoints, crucial for developing new treatments.

More Related Videos

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
08:46

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

7.0K
Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis
06:45

Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis

Published on: December 18, 2010

19.9K

Related Experiment Videos

Last Updated: Jan 29, 2026

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
14:54

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Published on: January 27, 2019

11.8K
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
08:46

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

7.0K
Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis
06:45

Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis

Published on: December 18, 2010

19.9K

Area of Science:

  • Infectious Diseases
  • Animal Models
  • Neonatal Research

Background:

  • Neonatal sepsis is a significant global health challenge.
  • Existing preclinical models lack standardization for screening interventions.
  • A robust model is essential for evaluating prophylactic and therapeutic strategies.

Purpose of the Study:

  • To detail a standardized preclinical model for neonatal polymicrobial sepsis in mice.
  • To establish clear protocols for sepsis induction and monitoring.
  • To define humane endpoints based on observable health criteria.

Main Methods:

  • Induction of sepsis in day-of-life 7 mice via intraperitoneal injection of cecal slurry.
  • Preparation of a homogeneous cecal slurry stock from pooled donors.
  • Weight- and litter-adjusted dosing and a defined monitoring schedule.
  • Health scoring based on righting reflex and mobility to determine humane endpoints.

Main Results:

  • The model allows for reproducible induction of neonatal sepsis.
  • Standardized slurry preparation minimizes donor variability and glycerol toxicity.
  • The monitoring strategy enables prediction of survival and early identification of humane endpoints.
  • Defined health categories (righting reflex, mobility) provide objective criteria for welfare assessment.

Conclusions:

  • This approach offers a standardized method for modeling neonatal sepsis in mice.
  • The defined humane endpoints enhance animal welfare and ethical considerations.
  • The model serves as a valuable tool for screening interventions against neonatal sepsis.