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Updated: Jan 29, 2026

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay
Published on: January 26, 2019
An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
Lien Anh Ha Do1, Reuben Tse2, Jordan Nathanielsz2
1Murdoch Children's Research Institute; Department of Paediatrics, University of Melbourne; lienanhha.do@mcri.edu.au.
Abstract:
Respiratory syncytial virus-specific neutralizing antibodies (RSV NAbs) are an important marker of protection against RSV. A number of different assay formats are currently in use worldwide so there is a need for an accurate and high-throughput method for measuring RSV NAbs. We describe here an imaging-based micro-neutralization assay that has been tested on RSV subgroup A and can also be adapted for RSV subgroup B and different sample types. This method is highly reproducible, with inter-assay variations for the reference antiserum being less than 10%. We believe this assay can be readily established in many laboratories worldwide at relatively low cost. Development of an improved, high-throughput assay that measures RSV NAbs represents a significant step forward for the standardization of this method internationally as well as being critical for the evaluation of novel RSV vaccine candidates in the future.
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