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Updated: Jan 29, 2026

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
Published on: July 24, 2021
CARS spectroscopy of Aspergillus nidulans spores
Benjamin D Strycker1,2, Zehua Han3, Blake Commer4
1Institute for Quantum Science and Engineering, Texas A&M University, College Station, Texas, USA. ben.strycker@tamu.edu.
Abstract:
Coherent Anti-Stokes Raman Spectroscopy (CARS) is performed on single spores (conidia) of the fungus Aspergillus nidulans in order to establish a baseline measurement for fungal spores. Chemical maps of single spores are generated and spectral differentiation between the cell wall and the cytoplasm is achieved. Principal Component Analysis of the measured spectra is then completed as a means to quantify spore heterogeneity. Applications range from the quick and accurate diagnosis of public health concerns to real-time agricultural and environmental sensing of fungal symbionts and pathogens.
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