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Responsive surface bioaffinity binding to construct flexible and sensitive electrochemical aptasensors.

Ying Liu1, Zhencai Zhu, Chao Wang

  • 1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China. sliu@qust.edu.cn.

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We developed a novel electrochemical aptasensor for sensitive detection of adenosine triphosphate (ATP) and thrombin. This biosensor offers a flexible and cost-effective platform for disease diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biosensors

Background:

  • Developing simple, flexible, cost-effective, and sensitive electrochemical biosensors is crucial for disease diagnostics and clinical biomedicine.
  • Existing amplified aptasensors often involve complex procedures that limit their widespread application.

Purpose of the Study:

  • To fabricate a novel enzyme-based electrochemical aptasensor using a responsive surface bioaffinity binding strategy.
  • To demonstrate the sensor's capability for sensitive detection of adenosine triphosphate (ATP) and thrombin.

Main Methods:

  • Fabrication of an electrochemical aptasensor utilizing a complex duplex probe (aptamer-hairpin and digoxigenin-labeled strand).
  • Exploitation of steric hindrance to control the binding of anti-digoxigenin-horseradish peroxidase (anti-Dig-HRP) antibody.
  • Target recognition triggers dissociation of the duplex probe, exposing digoxigenin for antibody binding and subsequent signal amplification via electrocatalysis.

Main Results:

  • Achieved sensitive detection of ATP with a limit of detection (LOD) of 0.87 nM.
  • Achieved sensitive detection of thrombin with a limit of detection (LOD) of 6.3 pM.
  • The proposed strategy demonstrated simplicity, sensitivity, and flexibility in biosensor fabrication.

Conclusions:

  • The developed aptasensor provides a simple, sensitive, and versatile platform for biosensing applications.
  • The responsive surface bioaffinity binding strategy offers a new pathway for fabricating advanced electrochemical biosensors.
  • This approach is promising for advancing disease diagnostics and clinical biomedicine.