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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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T Cell Types and Functions01:24

T Cell Types and Functions

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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Updated: Jan 29, 2026

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.

Krithika Hariharan1, Petra Reinke2,3, Andreas Kurtz4

  • 1Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité - Universitätsmedizin Berlin, Berlin, Germany. Krithika.hariharan@charite.de.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2019
PubMed
Summary

Researchers developed a protocol using human pluripotent stem cells (hPSCs) to generate various kidney cell types. These cells are valuable for drug testing, disease modeling, and regenerative medicine applications.

Keywords:
Directed differentiationKidneyKidney developmentNephrotoxicityPluripotent stem cells (PSCs)PodocytesProgenitorsTissue engineeringTubular epithelial cellsiPS

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Area of Science:

  • Stem cell biology
  • Nephrology
  • Regenerative Medicine

Background:

  • Human pluripotent stem cells (hPSCs) possess differentiation potential for various cell lineages.
  • Generating specific kidney cell types from hPSCs is crucial for research and therapeutic applications.

Purpose of the Study:

  • To detail a protocol for deriving segment-specific kidney cell types from hPSCs.
  • To provide a method for obtaining homogenous populations of kidney cells for downstream applications.

Main Methods:

  • Utilized a specific set of growth factors to induce kidney progenitor formation from hPSCs.
  • Further differentiated kidney progenitors in monolayer culture to yield distinct kidney cell types.
  • Detailed the step-by-step process for obtaining podocyte precursors, mesangial cells, and proximal/distal tubular epithelial cells, and collecting duct cells.

Main Results:

  • Successfully generated kidney progenitors from hPSCs.
  • Achieved differentiation into multiple, segment-specific kidney cell types including podocyte precursors, mesangial cells, and various tubular cells.
  • Obtained homogenous populations of these differentiated kidney cells.

Conclusions:

  • The developed protocol enables efficient generation of diverse kidney cell types from hPSCs.
  • These hPSC-derived kidney cells are suitable for nephrotoxicity testing, disease modeling, and tissue engineering.
  • This method advances the potential for in vitro kidney research and regenerative therapies.