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Updated: Jan 29, 2026

Purification of Endogenous Drosophila Transient Receptor Potential Channels
Published on: December 28, 2021
Simultaneous Visualization of Endogenous Homocysteine, Cysteine, Glutathione, and their Transformation through
Guoxing Yin1, Tingting Niu2, Ting Yu1
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province, Hunan Normal University, Changsha, 410081, China.
Abstract:
Studying numerous biologically important species simultaneously is crucial to understanding cellular functions and the root causes of related diseases. Direct visualization of endogenous biothiols in biological systems is of great value to understanding their biological roles. Herein, a novel multi-signal fluorescent probe was rationally designed and exploited for the simultaneous sensing of homocysteine (Hcy), cysteine (Cys), and glutathione (GSH) using different emission channels. This probe was successfully applied to the simultaneous discrimination between and visualization of endogenous Hcy, Cys, GSH, and their transformation in living cells.
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